A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing.
Stem Cell Res
; 45: 101837, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32413789
ABSTRACT
Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Nucleasas de los Efectores Tipo Activadores de la Transcripción
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Edición Génica
Límite:
Adult
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Animals
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Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article