A protein kinase A-regulated network encodes short- and long-lived cellular memories.
Sci Signal
; 13(632)2020 05 19.
Article
en En
| MEDLINE
| ID: mdl-32430291
ABSTRACT
Cells can store memories of prior experiences to modulate their responses to subsequent stresses, as seen for the protein kinase A (PKA)-mediated general stress response in yeast, which is required for resistance against future stressful conditions. Using microfluidics and time-lapse microscopy, we quantitatively analyzed how the cellular memory of stress adaptation is encoded in single yeast cells. We found that cellular memory was biphasic. Short-lived memory was mediated by trehalose synthase and trehalose metabolism. Long-lived memory was mediated by PKA-regulated stress-responsive transcription factors and cytoplasmic messenger ribonucleoprotein granules. Short- and long-lived memory could be selectively induced by different priming input dynamics. Computational modeling revealed how the PKA-mediated regulatory network could encode previous stimuli into memories with distinct dynamics. This biphasic memory-encoding scheme might represent a general strategy to prepare for future challenges in rapidly changing environments.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Quinasas Dependientes de AMP Cíclico
/
Proteínas de Saccharomyces cerevisiae
/
Redes Reguladoras de Genes
/
Modelos Biológicos
Idioma:
En
Año:
2020
Tipo del documento:
Article