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Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia.
Manning, Jessica E; Bohl, Jennifer A; Lay, Sreyngim; Chea, Sophana; Sovann, Ly; Sengdoeurn, Yi; Heng, Seng; Vuthy, Chan; Kalantar, Katrina; Ahyong, Vida; Tan, Michelle; Sheu, Jonathan; Tato, Cristina M; DeRisi, Joseph L; Baril, Laurence; Duong, Veasna; Dussart, Philippe; Karlsson, Erik A.
  • Manning JE; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
  • Bohl JA; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
  • Lay S; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
  • Chea S; Laboratory of Malaria and Vector Research, US National Institute of Allergy and Infectious Diseases, Phnom Penh, Cambodia.
  • Sovann L; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia.
  • Sengdoeurn Y; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia.
  • Heng S; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia.
  • Vuthy C; Cambodian Center for Disease Control, Ministry of Health, Phnom Penh, Cambodia.
  • Kalantar K; Chan Zuckerberg Initiative, Redwood City, California, USA.
  • Ahyong V; Chan Zuckerberg Biohub, San Francisco, California, USA.
  • Tan M; Chan Zuckerberg Biohub, San Francisco, California, USA.
  • Sheu J; Chan Zuckerberg Initiative, Redwood City, California, USA.
  • Tato CM; Chan Zuckerberg Biohub, San Francisco, California, USA.
  • DeRisi JL; Chan Zuckerberg Biohub, San Francisco, California, USA.
  • Baril L; Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
  • Duong V; Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
  • Dussart P; Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
  • Karlsson EA; Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
bioRxiv ; 2020 Mar 05.
Article en En | MEDLINE | ID: mdl-32511296
ABSTRACT
Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation sequencing on an iSeq100 Illumina platform paired with an open-source bioinformatics pipeline to quickly characterize Cambodia's first case of COVID-2019.
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