Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro.
Rev Bras Parasitol Vet
; 29(3): e012420, 2020 Aug 03.
Article
en En
| MEDLINE
| ID: mdl-32756775
ABSTRACT
Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
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Babesia
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Babesiosis
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Sangre
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Enfermedades de los Perros
Tipo de estudio:
Prognostic_studies
Límite:
Animals
País como asunto:
America do sul
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Brasil
Idioma:
En
Año:
2020
Tipo del documento:
Article