The use of Tween 20 alone as a blocking agent for immunoblotting can cause artefactual results.
J Immunol Methods
; 106(2): 175-9, 1988 Feb 10.
Article
en En
| MEDLINE
| ID: mdl-3276791
ABSTRACT
The use of Tween 20 as a suitable blocking agent in immunoblotting studies was evaluated by screening a panel of monoclonal antibodies (MoAbs) against a selection of blotted proteins which were unrelated to the antigens used to raise the MoAbs. Using Tween 20 alone to block the nitrocellulose membranes clear reactions were observed between the panel of MoAbs and several components of the blotted protein mixture. In contrast, when haemoglobin was used to block the membranes such reactions were not observed. In the absence of added protein the use of Tween 20 alone as a blocking agent for immunoblotting appears to lead to false positive reactions by non-specific antigen-antibody complexes.
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Banco de datos:
MEDLINE
Asunto principal:
Polisorbatos
/
Técnicas de Inmunoadsorción
/
Complejo Antígeno-Anticuerpo
/
Reacciones Antígeno-Anticuerpo
Idioma:
En
Año:
1988
Tipo del documento:
Article