Antibody Clicking as a Strategy to Modify Antibody Functionalities on the Surface of Targeted Cells.
J Am Chem Soc
; 142(37): 15644-15648, 2020 09 16.
Article
en En
| MEDLINE
| ID: mdl-32897068
ABSTRACT
We established a methodology for initiating cross-linking of antibodies selectively on the cell surface through intermolecular copper-free click reactions facilitated by increased effective concentrations of antibodies binding to target antigens. Upon cross-linking of tetrazine- and bicyclononyne-modified trastuzumab on the surface of HER2-overexpressing cells, increased antibody uptake and activation of intracellular signaling were observed. Our findings demonstrate that the cross-linking reaction can significantly alter the biophysical properties of proteins, activating their unique functionalities on targeted cells to realize an increased cargo delivery and synthetic manipulation of cellular signaling.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Aza
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Compuestos Bicíclicos con Puentes
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Reactivos de Enlaces Cruzados
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Trastuzumab
Límite:
Animals
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Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article