Your browser doesn't support javascript.
loading
Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore.
Tyson, John R; James, Phillip; Stoddart, David; Sparks, Natalie; Wickenhagen, Arthur; Hall, Grant; Choi, Ji Hyun; Lapointe, Hope; Kamelian, Kimia; Smith, Andrew D; Prystajecky, Natalie; Goodfellow, Ian; Wilson, Sam J; Harrigan, Richard; Snutch, Terrance P; Loman, Nicholas J; Quick, Joshua.
  • Tyson JR; Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
  • James P; Oxford Nanopore Technologies Ltd., Oxford, UK.
  • Stoddart D; Oxford Nanopore Technologies Ltd., Oxford, UK.
  • Sparks N; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
  • Wickenhagen A; MRC-University of Glasgow Centre for Virus Research, Glasgow, UK.
  • Hall G; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK.
  • Choi JH; Division of AIDS, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
  • Lapointe H; Division of AIDS, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
  • Kamelian K; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Smith AD; Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
  • Prystajecky N; Oxford Nanopore Technologies Ltd., Oxford, UK.
  • Goodfellow I; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
  • Wilson SJ; MRC-University of Glasgow Centre for Virus Research, Glasgow, UK.
  • Harrigan R; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK.
  • Snutch TP; Division of AIDS, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
  • Loman NJ; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Quick J; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
bioRxiv ; 2020 Sep 04.
Article en En | MEDLINE | ID: mdl-32908977
ABSTRACT
Genome sequencing has been widely deployed to study the evolution of SARS-CoV-2 with more than 90,000 genome sequences uploaded to the GISAID database. We published a method for SARS-CoV-2 genome sequencing (https//www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w) online on January 22, 2020. This approach has rapidly become the most popular method for sequencing SARS-CoV-2 due to its simplicity and cost-effectiveness. Here we present improvements to the original protocol i) an updated primer scheme with 22 additional primers to improve genome coverage, ii) a streamlined library preparation workflow which improves demultiplexing rate for up to 96 samples and reduces hands-on time by several hours and iii) cost savings which bring the reagent cost down to £10 per sample making it practical for individual labs to sequence thousands of SARS-CoV-2 genomes to support national and international genomic epidemiology efforts.
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Año: 2020 Tipo del documento: Article