D variants in the population of D-negative blood donors in the north-eastern region of Croatia.
Transfus Med
; 31(1): 43-47, 2021 Feb.
Article
en En
| MEDLINE
| ID: mdl-33034130
ABSTRACT
OBJECTIVES:
The aim of this study was to determine RHESUS D GENE (RHD) allelic variants among Croatian D-negative blood donors and compare our results with respective data from other European countries.BACKGROUND:
Altered or reduced D antigen expression can result in D variants, which can be mistyped and can lead to the alloimmunisation of the blood recipient. RHD genotyping can distinguish D variants weak D, partial D and DEL, thus preventing alloimmunisation. MATERIAL/METHODS:
A total of 6523 samples obtained from D-negative Croatian donors were screened for the presence of RHD using the real-time polymerase chain reaction (PCR) method. PCR-SSP was performed for D variant genotyping by using commercial genotyping kits (Inno-Train, Kronberg, Germany). Genomic DNA sequencing for all 10 exons of the RHD was performed when the genotyping kits failed to assign a D variant.RESULTS:
RHD molecular screening revealed 23 (0.35%) RHD-PCR positive samples, all C/E positive, in decreasing frequency 11 hybrid RHD-CE (2-9) D-CE variants, 4 weak partial D type 11 and 2 weak D type 2. Six samples remained unresolved and were sequenced. For 12 of 23 samples (excluding large hybrids), an adsorption/elution of anti-D serum was performed, confirming that all 12 were RhD+. The calculated frequency of clinically significant D alleles in RhD-negative blood donors was 1543 (0.18%) or 153 (1.89%) in C/E blood donors.CONCLUSION:
Data on the significant frequency of D variants among serologically D-negative blood donors in the north-eastern region of Croatia could help in introducing RHD molecular screening of blood donors in a routine workflow.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
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Donantes de Sangre
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Polimorfismo Conformacional Retorcido-Simple
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Técnicas de Genotipaje
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Genotipo
Límite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle aged
País como asunto:
Europa
Idioma:
En
Año:
2021
Tipo del documento:
Article