Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus.
J Clin Microbiol
; 59(1)2020 12 17.
Article
en En
| MEDLINE
| ID: mdl-33087429
ABSTRACT
We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Infecciones Estreptocócicas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
País como asunto:
America do norte
Idioma:
En
Año:
2020
Tipo del documento:
Article