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Expression Screening of Human Integral Membrane Proteins Using BacMam.
Mahajan, Pravin; Ellis, Katherine; Mukhopadhyay, Shubhashish; Fernandez-Cid, Alejandra; Chi, Gamma; Man, Henry; Dürr, Katharina L; Burgess-Brown, Nicola A.
  • Mahajan P; Astex Pharmaceuticals, Cambridge, UK.
  • Ellis K; Jenner Institute, University of Oxford, Oxford, UK.
  • Mukhopadhyay S; Structural Genomics Consortium, University of Oxford, Oxford, UK.
  • Fernandez-Cid A; Structural Genomics Consortium, University of Oxford, Oxford, UK.
  • Chi G; Structural Genomics Consortium, University of Oxford, Oxford, UK.
  • Man H; Structural Genomics Consortium, University of Oxford, Oxford, UK.
  • Dürr KL; Structural Genomics Consortium, University of Oxford, Oxford, UK.
  • Burgess-Brown NA; Structural Genomics Consortium, University of Oxford, Oxford, UK. nicola.burgess-brown@sgc.ox.ac.uk.
Methods Mol Biol ; 2199: 95-115, 2021.
Article en En | MEDLINE | ID: mdl-33125646
ABSTRACT
This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the BacMam system. This eukaryotic expression system was selected and a screening process established in 2016 to enable production of highly challenging human integral membrane proteins (IMPs), which are a significant component of our target list. Here, we discuss our recently developed platform for identifying expression and monodispersity of IMPs from 3 mL of HEK293 cells.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Expresión Génica / Vectores Genéticos / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Expresión Génica / Vectores Genéticos / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Año: 2021 Tipo del documento: Article