ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.
Sci Rep
; 10(1): 22031, 2020 12 16.
Article
en En
| MEDLINE
| ID: mdl-33328483
ABSTRACT
Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sondas de ADN
/
Hibridación Fluorescente in Situ
Límite:
Animals
/
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article