Hypoxia-cultured mouse mesenchymal stromal cells from bone marrow and compact bone display different phenotypic traits.
Exp Cell Res
; 399(1): 112434, 2021 02 01.
Article
en En
| MEDLINE
| ID: mdl-33340494
ABSTRACT
It has been suggested that the bone marrow microenvironment harbors two distinct populations of mesenchymal stromal cells (MSC), one with a perivascular location and other present in the endosteum. A better understanding of the biology of these MSC subsets has been pursued in order to refine its clinical application. However, most comparative characterizations of mouse MSC have been performed in normoxia. This can result in misleading interpretations since mouse MSC subsets with low/defective p53 activity are known to be selected during culture in normoxia. Here, we report a comprehensive in vitro characterization of mouse MSC isolated from bone marrow (BM-MSC) and compact bone (CB-MSC) expanded and assayed under hypoxia for their morphology, clonogenic efficiency and differentiation capacity. We found that, under hypoxia, compact bone is richer in absolute numbers of MSC and isolation of MSC from compact bone is associated with a reduced risk of hematopoietic cell carryover. In addition, CB-MSC have higher in vitro osteogenic capacity than BM-MSC, while adipogenic differentiation potential is similar. These findings reinforce the hypothesis of the existence of MSC in bone marrow and compact bone representing functionally distinct cell populations and highlight the compact bone as an efficient source of murine MSC under physiological oxygen concentrations.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células de la Médula Ósea
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Hipoxia de la Célula
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Células Madre Mesenquimatosas
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Hueso Cortical
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article