Beige adipocytes contribute to breast cancer progression.
Oncol Rep
; 45(1): 317-328, 2021 01.
Article
en En
| MEDLINE
| ID: mdl-33416183
ABSTRACT
Adipocytes are the main stromal cells in the mammary microenvironment, and crosstalk between adipocytes and breast cancer cells may play a critical and important role in cancer maintenance and progression. Tumorinduced differentiation to beige/brown adipose tissue is an important contribution to the hypermetabolic state of breast cancer. However, the effect of epithelial cellbeige adipocyte communication on tumor progression remains unclear. To contribute to the understanding of this phenomenon, we characterized components present in conditioned media (CM) from beige adipocytes (BAs) or white adipocytes (WAs), and evaluated the effects of BA and WACM on both adhesion and migration of tumor (LM3, 4T1 and MC4L1) and nontumor (NMuMG) mouse mammary epithelial cell lines. Additionally, we analyzed the expression of ObR, CD44, vimentin, MMP9, MCT1 and LDH in tumor and nontumor mouse mammary epithelial cell lines incubated with BACM, WACM or CtrolCM (control conditioned media). 3T3L1 preadipocytes differentiated into beige adipocytes upon PPARγ activation (rosiglitazone) displaying characteristics that morphologically resembled brown/beige adipocytes. Levels of UCP1, CIDEA, GLUT4, leptin, MCT4 and FABP4 were increased, while adiponectin, caveolin 1 and perilipin 1 levels were decreased in BAs with respect to WAs. Tumor cell lines revealed lower cell adhesion and increased cell migration after incubation with BA and WACM vs. CtrolCM. ObR and MMP9 in MC4L1 cells were significantly increased after incubation with BACM vs. WA and CtrolCM. In addition, MC4L1 and LM3 cells significantly increased their migration in the presence of BAs, suggesting that new signals originating from the crosstalk between BAs and tumor cells, could be responsible for this change. Our results indicate that beige adipocytes are able to regulate the behavior of both tumor and nontumor mouse mammary epithelial cells, favoring tumor progression.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
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Adipocitos Beige
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Neoplasias Mamarias Experimentales
Límite:
Animals
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Female
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article