Vaccinia Virus Infection Inhibits Skin Dendritic Cell Migration to the Draining Lymph Node.
J Immunol
; 206(4): 776-784, 2021 02 15.
Article
en En
| MEDLINE
| ID: mdl-33419767
ABSTRACT
There is a paucity of information on dendritic cell (DC) responses to vaccinia virus (VACV), including the traffic of DCs to the draining lymph node (dLN). In this study, using a mouse model of infection, we studied skin DC migration in response to VACV and compared it with the tuberculosis vaccine Mycobacterium bovis bacille Calmette-Guérin (BCG), another live attenuated vaccine administered via the skin. In stark contrast to BCG, skin DCs did not relocate to the dLN in response to VACV. Infection with UV-inactivated VACV or modified VACV Ankara promoted DC movement to the dLN, indicating that interference with skin DC migration requires replication-competent VACV. This suppressive effect of VACV was capable of mitigating responses to a secondary challenge with BCG in the skin, ablating DC migration, reducing BCG transport, and delaying CD4+ T cell priming in the dLN. Expression of inflammatory mediators associated with BCG-triggered DC migration were absent from virus-injected skin, suggesting that other pathways invoke DC movement in response to replication-deficient VACV. Despite adamant suppression of DC migration, VACV was still detected early in the dLN and primed Ag-specific CD4+ T cells. In summary, VACV blocks skin DC mobilization from the site of infection while retaining the ability to access the dLN to prime CD4+ T cells.
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Banco de datos:
MEDLINE
Asunto principal:
Piel
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Vaccinia
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Virus Vaccinia
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Células Dendríticas
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Movimiento Celular
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Ganglios Linfáticos
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article