Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).

Jones, Christopher M; Korosh, Travis C; Nielsen, David R; Pfleger, Brian F.

Jones CM; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA.
Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Nielsen DR; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA.
Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.

Appl Microbiol Biotechnol ; 105(3): 1147-1158, 2021 Feb.

Article en En | MEDLINE | ID: mdl-33443634

Asunto(s)

Synechococcus; ARN Polimerasas Dirigidas por ADN/genética; ARN Polimerasas Dirigidas por ADN/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Regulación Bacteriana de la Expresión Génica; Fenotipo; Synechococcus/genética; Synechococcus/metabolismo

Palabras clave

Cyanobacteria; Gene circuits; Promoters; Synthetic biology; T7-RNA polymerase