New C21-steroidal aglycones from the roots of Cynanchum otophyllum and their anticancer activity.
Fitoterapia
; 149: 104833, 2021 Mar.
Article
en En
| MEDLINE
| ID: mdl-33460724
ABSTRACT
Naturally occurring C21-steroidal aglycones from Cynanchum exhibit significant antitumor effects. To expand the chemical diversity and get large scale C21-steroidal aglycones, the extracts of the roots of Cynanchum otophyllum were treated with 5% HCl in aqueous and the resulting hydrolysate was investigated. Nine new C21-steroidal aglycones (1-9) namely cynotogenins A-I, along with seventeen known analogous (10-26), were isolated from the hydrolysate. The structures of compounds 1-9 were elucidated by spectroscopic analysis (IR, HR-ESI-MS, 1D and 2D NMR) and comparison of observed spectroscopic data with those of reported in the literature. Aglycones 2-5 with rare cis-cinnamoyl group as well as 8 and 9 with 5ß,6ß-epoxy group were found from the genus of Cynanchum for the first time. The cytotoxicities of compounds 1-26 toward human cancer HeLa, H1299, HepG2, and MCF-7 cells were evaluated and preliminary structure-activity relationship (SAR) was discussed. Moreover, compound 20 inhibits HepG2 cell apoptosis and induces of G0/G1 phase arrest in a dose dependent manner.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Esteroides
/
Cynanchum
/
Antineoplásicos Fitogénicos
Límite:
Humans
País como asunto:
Asia
Idioma:
En
Año:
2021
Tipo del documento:
Article