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Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy.
Li, Tianliang; Kong, Ligang; Li, Xinghui; Wu, Sijin; Attri, Kuldeep S; Li, Yan; Gong, Weipeng; Zhao, Bao; Li, Lupeng; Herring, Laura E; Asara, John M; Xu, Lei; Luo, Xiaobo; Lei, Yu L; Ma, Qin; Seveau, Stephanie; Gunn, John S; Cheng, Xiaolin; Singh, Pankaj K; Green, Douglas R; Wang, Haibo; Wen, Haitao.
  • Li T; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
  • Kong L; Shandong Institute of Otolaryngology, Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, China.
  • Li X; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
  • Wu S; College of Pharmacy, Medicinal Chemistry and Pharmacognosy, The Ohio State University, Columbus, OH, USA.
  • Attri KS; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA.
  • Li Y; Department of Physiology and Cell Biology, The Ohio State University, Columbus, OH, USA.
  • Gong W; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
  • Zhao B; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
  • Li L; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Herring LE; Proteomics Core Facility, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Asara JM; Division of Signal Transduction, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, MA, USA.
  • Xu L; Shandong Institute of Otolaryngology, Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, China.
  • Luo X; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Lei YL; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Ma Q; Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.
  • Seveau S; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
  • Gunn JS; Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.
  • Cheng X; College of Pharmacy, Medicinal Chemistry and Pharmacognosy, The Ohio State University, Columbus, OH, USA.
  • Singh PK; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA.
  • Green DR; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.
  • Wang H; Shandong Institute of Otolaryngology, Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, China. whboto11@163.com.
  • Wen H; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA. Haitao.Wen@osumc.edu.
Nat Microbiol ; 6(3): 366-379, 2021 03.
Article en En | MEDLINE | ID: mdl-33462436
ABSTRACT
Mitochondria are believed to have originated ~2.5 billion years ago. As well as energy generation in cells, mitochondria have a role in defence against bacterial pathogens. Despite profound changes in mitochondrial morphology and functions following bacterial challenge, whether intracellular bacteria can hijack mitochondria to promote their survival remains elusive. We report that Listeria monocytogenes-an intracellular bacterial pathogen-suppresses LC3-associated phagocytosis (LAP) by modulation of mitochondrial Ca2+ (mtCa2+) signalling in order to survive inside cells. Invasion of macrophages by L. monocytogenes induced mtCa2+ uptake through the mtCa2+ uniporter (MCU), which in turn increased acetyl-coenzyme A (acetyl-CoA) production by pyruvate dehydrogenase. Acetylation of the LAP effector Rubicon with acetyl-CoA decreased LAP formation. Genetic ablation of MCU attenuated intracellular bacterial growth due to increased LAP formation. Our data show that modulation of mtCa2+ signalling can increase bacterial survival inside cells, and highlight the importance of mitochondrial metabolism in host-microbial interactions.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Fagocitosis / Señalización del Calcio / Listeria monocytogenes / Proteínas Asociadas a Microtúbulos / Mitocondrias Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Fagocitosis / Señalización del Calcio / Listeria monocytogenes / Proteínas Asociadas a Microtúbulos / Mitocondrias Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Año: 2021 Tipo del documento: Article