Directional mast cell degranulation of tumor necrosis factor into blood vessels primes neutrophil extravasation.
Immunity
; 54(3): 468-483.e5, 2021 03 09.
Article
en En
| MEDLINE
| ID: mdl-33484643
ABSTRACT
Tissue resident mast cells (MCs) rapidly initiate neutrophil infiltration upon inflammatory insult, yet the molecular mechanism is still unknown. Here, we demonstrated that MC-derived tumor necrosis factor (TNF) was crucial for neutrophil extravasation to sites of contact hypersensitivity-induced skin inflammation by promoting intraluminal crawling. MC-derived TNF directly primed circulating neutrophils via TNF receptor-1 (TNFR1) while being dispensable for endothelial cell activation. The MC-derived TNF was infused into the bloodstream by directional degranulation of perivascular MCs that were part of the vascular unit with access to the vessel lumen. Consistently, intravenous administration of MC granules boosted neutrophil extravasation. Pronounced and rapid intravascular MC degranulation was also observed upon IgE crosslinking or LPs challenge indicating a universal MC potential. Consequently, the directional MC degranulation of pro-inflammatory mediators into the bloodstream may represent an important target for therapeutic approaches aimed at dampening cytokine storm syndromes or shock symptoms, or intentionally pushing immune defense.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Vasos Sanguíneos
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Factor de Necrosis Tumoral alfa
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Dermatitis por Contacto
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Inflamación
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Mastocitos
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Neutrófilos
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article