Nephroprotective effect of Artemisia herba alba aqueous extract in alloxan-induced diabetic rats.
J Tradit Complement Med
; 11(1): 53-61, 2021 Jan.
Article
en En
| MEDLINE
| ID: mdl-33511062
ABSTRACT
BACKGROUND AND AIM:
In the present study, we investigate the phytochemical composition and the nephroprotective effects as well as the antioxidant properties of Artemisia herba alba aqueous extract in alloxan-induced experimental diabetes in rats. EXPERIMENTAL PROCEDURE Wistar rats were divided into four groups of seven rats each Group I Normal control (NC) received saline solution at 9 given by intraperitoneal way; Group II Diabetic control (DC) received alloxan (150 mg/kg b.w) intraperitoneally; Group III Normal control (NC + AHA) received saline solution at 9 and treated orally by AHA aqueous extract (400 mg/kg/b.w); Group IV Diabetic control (DC + AHA) received alloxan solution (150 mg/kg b.w) intraperitoneally and treated by aqueous extract of AHA (400 mg/kg/b.w/day) orally after one week of alloxan administration. After 30 days, blood and tissue samples were collected for biochemical and histopathological analysis, respectively. Glomerular damage markers, including creatinine, serum urea, urine creatinine and urine urea levels were estimated. Creatinine clearance was also assessed. Oxidative stress parameters were assessed in the kidney homogenate. RESULTS ANDCONCLUSION:
Alloxan-exposure resulted in significant increase in blood glucose and serum level of glomerular damage markers. The antioxidant enzyme activities were significantly downregulated associated with an increase in malondialdehyde (MDA) level over the baseline values. Artemisia herba alba aqueous extract supplementation significantly improved the studied parameters. In concluding, the results obtained suggests that Artemisia herbs-alba aqueous extract supplementation reduces alloxan-induced free radical generation, potentiates the antioxidant defense system and alleviates renal sensitivity to oxidative stress.
AHA, Artemisia herba-alba; AlCl3, Aluminum trichloride; Artemisia herba alba; CAT, catalase; DC, Diabetic control; DPPH, 1,1-diphenyl-2-picrylhydrazyl; DTNB, 5,5-dithiobis (2-nitrobenzoic acid); Diabetes; Free radicals; GPx, glutathione peroxidase; GSH, reduced glutathione; GST, glutathione-S-transferase; H2O2, hydrogen peroxide; MDA, malondialdehyde; NBT, Nitro-blue tetrazolium; Nephroprotection; Oxidative stress; RFC, Folin-Ciocalteu; ROS, reactive oxygen species; SOD, superoxide dismutase; STZ, streptozotocin; TBA, thiobarbituric acid; TCA, trichloroacetic acid
Texto completo:
1
Banco de datos:
MEDLINE
Idioma:
En
Año:
2021
Tipo del documento:
Article