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A simple ATAC-seq protocol for population epigenetics.
Augusto, Ronaldo de Carvalho; Rey, Oliver; Cosseau, Céline; Chaparro, Cristian; Vidal-Dupiol, Jérémie; Allienne, Jean-François; Duval, David; Pinaud, Silvain; Tönges, Sina; Andriantsoa, Ranja; Luquet, Emilien; Aubret, Fabien; Dia Sow, Mamadou; David, Patrice; Thomson, Vicki; Joly, Dominique; Gomes Lima, Mariana; Federico, Déborah; Danchin, Etienne; Minoda, Aki; Grunau, Christoph.
  • Augusto RC; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Rey O; LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France.
  • Cosseau C; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Chaparro C; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Vidal-Dupiol J; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Allienne JF; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Duval D; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Pinaud S; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Tönges S; Univ. Montpellier, CNRS, IFREMER, UPVD, IHPE, F-66000 Perpignan and F-34095, Montpellier, France.
  • Andriantsoa R; Cancer Research UK, Cambridge Institute, University of Cambridge, Cambridge, UK.
  • Luquet E; Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, 69120, Germany.
  • Aubret F; Division of Epigenetics, DKFZ ZMBH Alliance, German Cancer Research Center, Heidelberg, 69120, Germany.
  • Dia Sow M; Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Villeurbanne, 69622, France.
  • David P; CNRS,Station d'Ecologie Théorique et Expérimentale, Université Paul Sabatier, Moulis, 09200, France.
  • Thomson V; School of Molecular and Life Sciences, Curtin University, Bentley, Australia.
  • Joly D; LBLGC, INRA, Université d'Orléans, Orléans, France.
  • Gomes Lima M; Univ. Montpellier, CNRS, CEFE, F-34293, Montpellier, France.
  • Federico D; School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia.
  • Danchin E; Laboratoire Evolution, Génomes Comportement, Ecologie, CNRS Université Paris Sud UMR 9191, Gif sur Yvette, 91198, France.
  • Minoda A; Laboratório de Malacologia, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, RJ, Brazil.
  • Grunau C; Laboratoire Évolution & Diversité Biologique (EDB UMR 5174), Université Fédérale de Toulouse; CNRS, Toulouse, 31062, France.
Wellcome Open Res ; 5: 121, 2020.
Article en En | MEDLINE | ID: mdl-33521328
ABSTRACT
We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size, and it requires little molecular biology handling expertise.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2020 Tipo del documento: Article