Engineering 3D genome organization.
Nat Rev Genet
; 22(6): 343-360, 2021 06.
Article
en En
| MEDLINE
| ID: mdl-33558716
ABSTRACT
Cancers and developmental disorders are associated with alterations in the 3D genome architecture in space and time (the fourth dimension). Mammalian 3D genome organization is complex and dynamic and plays an essential role in regulating gene expression and cellular function. To study the causal relationship between genome function and its spatio-temporal organization in the nucleus, new technologies for engineering and manipulating the 3D organization of the genome have been developed. In particular, CRISPR-Cas technologies allow programmable manipulation at specific genomic loci, enabling unparalleled opportunities in this emerging field of 3D genome engineering. We review advances in mammalian 3D genome engineering with a focus on recent manipulative technologies using CRISPR-Cas and related technologies.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ingeniería Genética
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Núcleo Celular
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Genoma
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Genómica
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Sistemas CRISPR-Cas
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Edición Génica
Límite:
Animals
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article