Detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR: A better predictor of bacterial vaginosis.
Anaerobe
; 69: 102343, 2021 Jun.
Article
en En
| MEDLINE
| ID: mdl-33582302
ABSTRACT
The present study aimed to determine the utility of detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR for diagnosing bacterial vaginosis (BV). Vaginal samples (n = 385) categorized as positive (n = 108) or negative (n = 208) for bacterial vaginosis based on the Nugent scoring system, were analyzed for the presence of G. vaginalis and A. vaginae by conventional PCR. We compared the sensitivity, specificity, positive predictive value, negative predictive value and odds ratio for the detection of each bacterium alone with the combination of the two bacteria for diagnosing BV. The detection of co-infection of the two bacteria demonstrated a sensitivity of 96%, a specificity of 82.9%, a positive predictive value of 68.5%, a negative predictive value of 98.2% with an odds ratio of 116 (CI -32 - 409). In our study, we found a high sensitivity, specificity, negative predictive value and odds ratio for the detection of co-infection of A. vaginae and G. vaginalis for the diagnosis of BV.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Gardnerella vaginalis
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Reacción en Cadena de la Polimerasa
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Vaginosis Bacteriana
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Infecciones por Bacterias Grampositivas
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Actinobacteria
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Coinfección
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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Qualitative_research
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Risk_factors_studies
Límite:
Adolescent
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Adult
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Female
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article