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Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria.
Daodu, O B; Shaibu, J O; Richards, A B; Folaranmi, E B; Adegoke, S; Ajadi, A; Olorunshola, I D; Akanbi, O B; Afolabi, A A; Daodu, O C; Aiyedun, J O; Oludairo, O O; Halleed, N I; Audu, R A; Oluwayelu, D O.
  • Daodu OB; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. daodu.ob@unlorin.edu.ng.
  • Shaibu JO; Center for Human Virology and Genomics, Nigerian Institute for Medical Research, Yaba, Lagos State, Nigeria.
  • Richards AB; Kwara State Ministry of Agriculture and Rural Development, Ilorin, Kwara State, Nigeria.
  • Folaranmi EB; Kwara State Ministry of Agriculture and Rural Development, Ilorin, Kwara State, Nigeria.
  • Adegoke S; Kwara State Ministry of Agriculture and Rural Development, Ilorin, Kwara State, Nigeria.
  • Ajadi A; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Olorunshola ID; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Akanbi OB; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Afolabi AA; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Daodu OC; Department of Wildlife and Ecotourism, Faculty of Agriculture and Forestry, University of Ibadan, Ibadan, Nigeria.
  • Aiyedun JO; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Oludairo OO; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.
  • Halleed NI; Kwara State Ministry of Agriculture and Rural Development, Ilorin, Kwara State, Nigeria.
  • Audu RA; Center for Human Virology and Genomics, Nigerian Institute for Medical Research, Yaba, Lagos State, Nigeria.
  • Oluwayelu DO; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
Trop Anim Health Prod ; 53(1): 185, 2021 Feb 28.
Article en En | MEDLINE | ID: mdl-33641034
ABSTRACT
Rabbit haemorrhagic disease virus (RHDV) was recovered from necropsied rabbits that died during an outbreak characterized by epistaxis, incoordination, paralysis, and multi-organ haemorrhages in Ilorin, Nigeria. The haemagglutination test (HA) and RT-PCR assay targeted against a fragment of the RHDV VP60 gene were performed on liver, spleen, and kidney homogenates; faeces; and urine obtained from the rabbits. Amplicons were purified, sequenced, and phylogenetically analysed. The liver homogenates yielded the highest HA titres while RT-PCR of liver, spleen, and kidneys yielded the expected 1252 bp band. Sequence and phylogenetic analyses revealed that the Nigerian RHDV strain (RHDV/NGR/ILN/001) was 98.57%, 97.95%, and 96.70% homologous with RHDV2 (RHDVGI.2) strains from the Netherlands, Germany, and France, respectively. RHDV/NGR/ILN/001 induced tracheal, intestinal, and mediastinal lymph node haemorrhages, pulmonary oedema and congestion, and enlarged, necrotic liver in experimentally inoculated rabbits. The implications of this study, which is the first report of RHDV in Nigeria, are discussed.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de la Enfermedad Hemorrágica del Conejo / Infecciones por Caliciviridae Tipo de estudio: Diagnostic_studies Límite: Animals País como asunto: Africa Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de la Enfermedad Hemorrágica del Conejo / Infecciones por Caliciviridae Tipo de estudio: Diagnostic_studies Límite: Animals País como asunto: Africa Idioma: En Año: 2021 Tipo del documento: Article