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Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds.
Rafael, Míriam Silva; Bridi, Leticia Cegatti; Sharakhov, Igor V; Marinotti, Osvaldo; Sharakhova, Maria V; Timoshevskiy, Vladimir; Guimarães-Marques, Giselle Moura; Santos, Valéria Silva; da Silva, Carlos Gustavo Nunes; Astolfi-Filho, Spartaco; Tadei, Wanderli Pedro.
  • Rafael MS; Coordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, Brazil.
  • Bridi LC; Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil.
  • Sharakhov IV; Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil.
  • Marinotti O; Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • Sharakhova MV; Laboratory of Evolutionary Genomics of Insects, the Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Timoshevskiy V; Department of Genetics and Cell Biology, Tomsk State University, 634050 Tomsk, Russia.
  • Guimarães-Marques GM; MTEKPrime, Aliso Viejo, CA 92656, USA.
  • Santos VS; Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • da Silva CGN; Laboratory of Evolutionary Genomics of Insects, the Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
  • Astolfi-Filho S; Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • Tadei WP; Department of Biology, University of Kentucky, Lexington, KY 40506, USA.
Insects ; 12(2)2021 Feb 15.
Article en En | MEDLINE | ID: mdl-33671870
ABSTRACT
The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2021 Tipo del documento: Article