The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal.
Sci Rep
; 11(1): 5358, 2021 03 08.
Article
en En
| MEDLINE
| ID: mdl-33686165
ABSTRACT
The morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the outer nuclear membrane nesprin proteins, which comprise the LINC complex. We found that depletion of SUN1 leads to Golgi complex dispersion with maintenance of ministacks and retained function for vesicle transport through the Golgi complex. In addition, SUN2 associates with microtubule plus-end-directed motor KIF20A, possibly via nesprin-2. KIF20A plays a role in the Golgi dispersion in conjunction with the SUN2-nesprin-2 LINC complex in SUN1-depleted cells, suggesting that SUN1 suppresses the function of the SUN2-nesprin-2 LINC complex under a steady-state condition. Further, SUN1-knockout mice, which show impaired cerebellar development and cerebellar ataxia, presented altered Golgi morphology in Purkinje cells. These findings revealed a regulation of the Golgi organization by the LINC complex.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
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Cinesinas
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Proteínas de Unión a Telómeros
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Complejos Multiproteicos
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Péptidos y Proteínas de Señalización Intracelular
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Aparato de Golgi
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Proteínas de la Membrana
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Proteínas de Microfilamentos
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Proteínas del Tejido Nervioso
Límite:
Animals
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article