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The Netrin-1-Neogenin-1 signaling axis controls neuroblastoma cell migration via integrin-ß1 and focal adhesion kinase activation.
Villanueva, Andrea A; Sanchez-Gomez, Pilar; Muñoz-Palma, Ernesto; Puvogel, Sofía; Casas, Bárbara S; Arriagada, Cecilia; Peña-Villalobos, Isaac; Lois, Pablo; Ramírez Orellana, Manuel; Lubieniecki, Fabiana; Casco Claro, Fernando; Gallegos, Iván; García-Castro, Javier; Torres, Vicente A; Palma, Verónica.
  • Villanueva AA; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
  • Sanchez-Gomez P; Neurooncology Unit, Unidad Funcional de Investigación en Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
  • Muñoz-Palma E; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
  • Puvogel S; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
  • Casas BS; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
  • Arriagada C; Institute for Research in Dental Sciences, Faculty of Dentistry, Universidad de Chile, Olivos 943, Independencia, Santiago, Chile.
  • Peña-Villalobos I; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
  • Lois P; Postgraduate in Education Department, Faculty of Humanities, Universidad Mayor. Santiago, Chile.
  • Ramírez Orellana M; Postgraduate in Education Department, Faculty of Humanities, Universidad Mayor. Santiago, Chile.
  • Lubieniecki F; Hospital de Pediatría Dr. Prof. Juan P. Garrahan, Buenos Aires, Argentina.
  • Casco Claro F; Unidad Anatomía Patológica, Unilabs, Madrid, España.
  • Gallegos I; Universidad de Chile, Universidad de Chile, Santiago, Chile.
  • García-Castro J; Cellular Biotechnology Unit, Instituto de Investigación de Enfermedades Raras (IIER), Instituto de Salud Carlos III, ISCIII, Madrid, Spain.
  • Torres VA; Institute for Research in Dental Sciences, Faculty of Dentistry, Universidad de Chile, Olivos 943, Independencia, Santiago, Chile.
  • Palma V; Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences. Universidad de Chile, Santiago, Chile.
Cell Adh Migr ; 15(1): 58-73, 2021 12.
Article en En | MEDLINE | ID: mdl-33724150
ABSTRACT
Neuroblastoma is a highly metastatic tumor that emerges from neural crest cell progenitors. Focal Adhesion Kinase (FAK) is a regulator of cell migration that binds to the receptor Neogenin-1 and is upregulated in neuroblastoma. Here, we show that Netrin-1 ligand binding to Neogenin-1 leads to FAK autophosphorylation and integrin ß1 activation in a FAK dependent manner, thus promoting neuroblastoma cell migration. Moreover, Neogenin-1, which was detected in all tumor stages and was required for neuroblastoma cell migration, was found in a complex with integrin ß1, FAK, and Netrin-1. Importantly, Neogenin-1 promoted neuroblastoma metastases in an immunodeficient mouse model. Taken together, these data show that Neogenin-1 is a metastasis-promoting protein that associates with FAK, activates integrin ß1 and promotes neuroblastoma cell migration.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Integrina beta1 / Neuroblastoma Límite: Animals Idioma: En Año: 2021 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Integrina beta1 / Neuroblastoma Límite: Animals Idioma: En Año: 2021 Tipo del documento: Article