MicroELISA detection of thymosin alpha 1 released in thymic organ cultures.

Sinopoli, N T; Pesce, A; Baldassarre, A M; Trivers, G E; Frati, L; Rinaldi-Garaci, C

Sinopoli, N T; Pesce, A; Baldassarre, A M; Trivers, G E; Frati, L; Rinaldi-Garaci, C.

Sinopoli NT; Department of Experimental Medicine, 1st Rome University, Italy.

J Immunol Methods ; 110(2): 261-5, 1988 Jun 13.

Article en En | MEDLINE | ID: mdl-3379314

ABSTRACT

ABSTRACT

Using a polyclonal specific rabbit anti-thymosin alpha 1 a highly sensitive enzyme-linked immunosorbent assay (ELISA) has been developed to measure thymosin alpha 1. Production of thymosin alpha 1 was detected in both thymic organ cultures and in mouse serum. The method is rapid (5 h), reproducible and easy to perform.

Asunto(s)

Ensayo de Inmunoadsorción Enzimática; Timosina/análogos & derivados; Timo/metabolismo; Animales; Reacciones Antígeno-Anticuerpo; Hígado/metabolismo; Masculino; Ratones; Ratones Endogámicos C57BL; Técnicas de Cultivo de Órganos; Bazo/metabolismo; Timalfasina; Timosina/análisis; Timosina/inmunología; Timosina/metabolismo; Timo/citología

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Banco de datos: MEDLINE Asunto principal: Timosina / Timo / Ensayo de Inmunoadsorción Enzimática Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Año: 1988 Tipo del documento: Article

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