Comparative in vitro study on the local tolerance and efficacy of benzalkonium chloride, myristalkonium chloride and nonoxynol-9 as active principles in vaginal contraceptives.
Eur J Contracept Reprod Health Care
; 26(4): 334-342, 2021 Aug.
Article
en En
| MEDLINE
| ID: mdl-33821738
ABSTRACT
BACKGROUND:
Spermicides have been identified as a potentially attractive alternative to hormonal contraceptives and/or intrauterine devices. Thus, this study aimed evaluating the efficacy and local tolerance of benzalkonium chloride (BKC) and myristalkonium chloride (MKC) contained in Pharmatex® vaginal formulations and compare them with nonoxynol-9 (N-9), the most common active ingredient in topical vaginal contraceptives.METHODS:
Human normozoospermic samples were assessed for motility, viability, acrosome status and penetration ability after exposure to control, N-9 or different BKC and MKC doses for 0 and 10 minutes. Local tolerance on HeLa cells was evaluated by the Trypan-blue and MTT assays.RESULTS:
Exposure to BKC and MKC reduced acrosome integrity while promoting total immobilisation and complete loss of sperm viability (p < .001, n = 15). Both compounds also compromised sperm penetration ability upon exposure (p < .001, n = 15). N-9 induced the same outcomes (p < .001, n = 15); nevertheless, it was more toxic to HeLa cells than BKC and MKC (p < .05, n = 14).CONCLUSIONS:
BKC and MKC present strong in vitro spermicidal activity at lower doses than N-9 and were better tolerated after immediate exposure than N-9. Available Pharmatex® galenic formulations were as effective as products based on N-9.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espermicidas
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Espermatozoides
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Compuestos de Benzalconio
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Nonoxinol
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Anticonceptivos
Límite:
Female
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Humans
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Male
Idioma:
En
Año:
2021
Tipo del documento:
Article