Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography.
STAR Protoc
; 2(2): 100411, 2021 06 18.
Article
en En
| MEDLINE
| ID: mdl-33870220
ABSTRACT
RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the method and present two approaches one that includes RNA isolation for maximum sensitivity and one that is suitable for partial automatization and is therefore less time-consuming. For complete details on the use and execution of this protocol, please refer to Ebbing et al. (2018).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tomografía
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Caenorhabditis elegans
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Perfilación de la Expresión Génica
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Análisis de la Célula Individual
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article