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3-Benzoylquinoxalinone as a photoaffinity labelling derivative with fluorogenic properties allowing reaction monitoring under "no-wash" conditions.
Cauwel, Madeleine; Guillou, Clément; Renault, Kévin; Schapman, Damien; Bénard, Magalie; Galas, Ludovic; Cosette, Pascal; Renard, Pierre-Yves; Sabot, Cyrille.
  • Cauwel M; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, COBRA (UMR 6014), Rouen 76000, France. cyrille.sabot@univ-rouen.fr.
  • Guillou C; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, PBS-UMR6270 CNRS, PISSARO Proteomics Facility, IRIB, Rouen 76000, France.
  • Renault K; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, COBRA (UMR 6014), Rouen 76000, France. cyrille.sabot@univ-rouen.fr.
  • Schapman D; Normandie Univ, Inserm, UNIROUEN, PRIMACEN, Cell Imaging Platform of Normandy, IRIB, Rouen 76000, France.
  • Bénard M; Normandie Univ, Inserm, UNIROUEN, PRIMACEN, Cell Imaging Platform of Normandy, IRIB, Rouen 76000, France.
  • Galas L; Normandie Univ, Inserm, UNIROUEN, PRIMACEN, Cell Imaging Platform of Normandy, IRIB, Rouen 76000, France.
  • Cosette P; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, PBS-UMR6270 CNRS, PISSARO Proteomics Facility, IRIB, Rouen 76000, France.
  • Renard PY; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, COBRA (UMR 6014), Rouen 76000, France. cyrille.sabot@univ-rouen.fr.
  • Sabot C; Normandie Univ, CNRS, UNIROUEN, INSA Rouen, COBRA (UMR 6014), Rouen 76000, France. cyrille.sabot@univ-rouen.fr.
Chem Commun (Camb) ; 57(32): 3893-3896, 2021 Apr 25.
Article en En | MEDLINE | ID: mdl-33871509
ABSTRACT
Described herein is a quinoxalinone-based photoaffinity probe with caged fluorescence properties. Upon visible blue LED irradiation (λmax 450 nm), this photo-crosslinker is able to covalently capture proteins with concomitant fluorescence labelling. This process enables monitoring applications under "no wash" conditions.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2021 Tipo del documento: Article