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Chronic cannabidiol (CBD) administration induces anticonvulsant and antiepileptogenic effects in a genetic model of epilepsy.
Lazarini-Lopes, Willian; Do Val-da Silva, Raquel A; da Silva-Júnior, Rui M P; Silva-Cardoso, Gleice K; Leite-Panissi, Christie R A; Leite, João P; Garcia-Cairasco, Norberto.
  • Lazarini-Lopes W; Neuroscience and Behavioral Sciences Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil; Neurophysiology and Experimental Neuroethology Laboratory, Physiology Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil. E
  • Do Val-da Silva RA; Neuroscience and Behavioral Sciences Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil.
  • da Silva-Júnior RMP; Neurophysiology and Experimental Neuroethology Laboratory, Physiology Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: ruimiltonpsj@usp.br.
  • Silva-Cardoso GK; Psychology Department, Faculty of Phylosophy, Science, and Letters, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: cardoso.gkrs@usp.br.
  • Leite-Panissi CRA; Psychology Department, Faculty of Phylosophy, Science, and Letters, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: christie@usp.br.
  • Leite JP; Neuroscience and Behavioral Sciences Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: jpleite@fmrp.usp.br.
  • Garcia-Cairasco N; Neuroscience and Behavioral Sciences Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil; Neurophysiology and Experimental Neuroethology Laboratory, Physiology Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, Brazil. E
Epilepsy Behav ; 119: 107962, 2021 06.
Article en En | MEDLINE | ID: mdl-33887676
ABSTRACT
Cannabidiol (CBD) is a marijuana compound implicated in epilepsy treatment in animal models and pharmacoresistant patients. However, little is known about chronic CBD administration's effects in chronic models of seizures, especially regarding its potential antiepileptogenic effects. In the present study, we combined a genetic model of epilepsy (the Wistar Audiogenic Rat strain - WARs), a chronic protocol of seizures (the audiogenic kindling - AuK), quantitative and sequential behavioral analysis (neuroethology), and microscopy imaging to analyze the effects of chronic CBD administration in a genetic model of epilepsy. The acute audiogenic seizure is characterized by tonic-clonic seizures and intense brainstem activity. However, during the AuK WARs can develop limbic seizures associated with the recruitment of forebrain and limbic structures. Here, chronic CBD administration, twice a day, attenuated brainstem, tonic-clonic seizures, prevented limbic recruitment, and suppressed limbic (kindled) seizures, suggesting CBD antiepileptogenic effects. Additionally, CBD prevented chronic neuronal hyperactivity, suppressing FosB immunostaining in the brainstem (inferior colliculus and periaqueductal gray matter) and forebrain (basolateral amygdala nucleus and piriform cortex), structures associated with tonic-clonic and limbic seizures, respectively. Chronic seizures increased cannabinoid receptors type 1 (CB1R) immunostaining in the hippocampus and the BLA, while CBD administration prevented changes in CB1R expression induced by the AuK. The neuroethological analysis provided details about CBD's protective effects against brainstem and limbic seizures associated with FosB expression. Our results strongly suggest chronic CBD anticonvulsant and antiepileptogenic effects associated with reduced chronic neuronal activity and modulation of CB1R expression. We also support the chronic use of CBD for epilepsies treatments.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cannabidiol / Anticonvulsivantes Tipo de estudio: Guideline Límite: Animals / Humans Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cannabidiol / Anticonvulsivantes Tipo de estudio: Guideline Límite: Animals / Humans Idioma: En Año: 2021 Tipo del documento: Article