Exogenous ATP in the Cryopreservation of Brycon orbignyanus Spermatozoa.
Cryo Letters
; 41(1): 13-18, 2020.
Article
en En
| MEDLINE
| ID: mdl-33973979
ABSTRACT
BACKGROUND:
ATP exogenous (ATPe) has been used successfully in improving motility and fertility for many animal species. However this has not yet been tested on Brycon orbignyamus.OBJECTIVE:
The objective of this study was to evaluate the use of ATPe for the cryopreservation of sperm from B. orbignyamus. MATERIALS ANDMETHODS:
The ATPe concentrations tested were 1.0 µM, 5.0 µM and 10 µM combined with Beltsville Thawing SolutionTM extender and dimethylformamide at 7.5%. The sperm were frozen in a nitrogen vapour vessel and stored in liquid nitrogen at -196 ºC. The parameters of viability post-thawing were evaluated using CASA, and flow cytometer.RESULTS:
The ATPe did not promote improvements in spermatic kinetics, and in the higher concentrations caused a worsening in these parameters. Also there was loss of mitochondrial functionality and greater cellular disruption with the concentration of 10 µM.CONCLUSION:
We do not recommend the addition of ATP for cryopreserving B. orbignyamus.
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Banco de datos:
MEDLINE
Asunto principal:
Preservación de Semen
/
Criopreservación
/
Adenosina Trifosfato
/
Crioprotectores
/
Characiformes
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article