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Extract of white sweet potato tuber against TNF-α-induced insulin resistance by activating the PI3K/Akt pathway in C2C12 myotubes.
Shyur, Lie-Fen; Varga, Viola; Chen, Chiao-Ming; Mu, Shu-Chi; Chang, Yu-Chih; Li, Sing-Chung.
  • Shyur LF; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 11529, Taiwan.
  • Varga V; School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan.
  • Chen CM; Institute of Translational Medicine, Semmelweis University, 1094, Budapest, Hungary.
  • Mu SC; Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei, 10462, Taiwan.
  • Chang YC; School of Medicine, Fu-Jen Catholic University, New Taipei City, 24205, Taiwan.
  • Li SC; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
Bot Stud ; 62(1): 7, 2021 May 18.
Article en En | MEDLINE | ID: mdl-34003397
ABSTRACT

BACKGROUND:

White sweet potato (WSP; Ipomoea batatas L. Simon No. 1) has many potential beneficial effects on metabolic control and diabetes-related insulin resistance. The improvement of insulin resistance by WSP tuber extracts on glucose uptake were not investigated in C2C12 myoblast cells.

RESULTS:

WSP tuberous ethanol extract (WSP-E) was partitioned with ethyl-acetate and water to obtain ethyl-acetate layer (WSP-EA) and water layer (WSP-EW). The WSP-EA shows the highest total phenolic contents and highest antioxidant activity by Folin-Ciocalteu and (2,2-diphenyl-1-picryl-hydrazyl-hydrate, DPPH) assay, respectively. After low concentration horse serum on differentiation inducement of C2C12 myoblasts into mature myotubes, the cells were treated with TNF-α to induce insulin resistance. WSP-EA and WSP-EW extracts increased the uptake of fluorescence glucose analogue (2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy-D-glucose, 2-NBDG) in a dose-dependent manner as examined by flow cytometry. The WSP-EA enhanced glucose uptake by activation of phosphorylation of IR (pIR), IRS-1 (pIRS-1) and Akt (pAkt) involved in PI3K (phosphatidylinositol 3-kinase)/protein kinase B (Akt) pathway, also upregulated glucose transporter 4 (GLUT4) expression in myotubes.

CONCLUSIONS:

WSP-EA enhanced the glucose uptake in C2C12 myotubes through upregulating the PI3K/Akt pathway. The in vitro data reveal that WSP tuber extracts has potential applications to improve insulin resistance in diabetes.
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