Native mass spectrometry and gas-phase fragmentation provide rapid and in-depth topological characterization of a PROTAC ternary complex.

Song, Jong Hee; Wagner, Nicole D; Yan, Jing; Li, Jing; Huang, Richard Y-C; Balog, Aaron J; Newitt, John A; Chen, Guodong; Gross, Michael L

Song, Jong Hee; Wagner, Nicole D; Yan, Jing; Li, Jing; Huang, Richard Y-C; Balog, Aaron J; Newitt, John A; Chen, Guodong; Gross, Michael L.

Song JH; Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Wagner ND; Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Yan J; Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Li J; Bristol Myers Squibb Company, Research and Early Development, Princeton, NJ 08543, USA.
Huang RY; Bristol Myers Squibb Company, Research and Early Development, Princeton, NJ 08543, USA.
Balog AJ; Bristol Myers Squibb Company, Research and Early Development, Princeton, NJ 08543, USA.
Newitt JA; Bristol Myers Squibb Company, Research and Early Development, Princeton, NJ 08543, USA.
Chen G; Bristol Myers Squibb Company, Research and Early Development, Princeton, NJ 08543, USA.
Gross ML; Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA. Electronic address: mgross@wustl.edu.

Cell Chem Biol ; 28(10): 1528-1538.e4, 2021 10 21.

Article en En | MEDLINE | ID: mdl-34081921

Asunto(s)

Proteínas de Ciclo Celular/metabolismo; Gases/química; Espectrometría de Movilidad Iónica/métodos; Factores de Transcripción/metabolismo; Ubiquitina-Proteína Ligasas/metabolismo; Proteínas de Ciclo Celular/química; Mapas de Interacción de Proteínas; Proteolisis; Factores de Transcripción/química; Ubiquitina-Proteína Ligasas/química

Palabras clave

CID (collision-induced dissociation); CIU (collision-induced unfolding); E3 ligase; ECD (electron capture dissociation); IMS (ion mobility spectrometry); MZ1; PROTACs (proteolysis-targeting chimeras); charge-reducing agent; native mass spectrometry; ternary complex

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Factores de Transcripción / Proteínas de Ciclo Celular / Ubiquitina-Proteína Ligasas / Espectrometría de Movilidad Iónica / Gases Tipo de estudio: Prognostic_studies Idioma: En Año: 2021 Tipo del documento: Article

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