Vinyl-Fluorene Molecular Wires for Voltage Imaging with Enhanced Sensitivity and Reduced Phototoxicity.
J Am Chem Soc
; 143(31): 11903-11907, 2021 08 11.
Article
en En
| MEDLINE
| ID: mdl-34323478
ABSTRACT
Fluorescent voltage indicators are an attractive alternative for studying the electrical activity of excitable cells; however, the development of indicators that are both highly sensitive and low in toxicity over long-term experiments remains a challenge. Previously, we reported a fluorene-based voltage-sensitive fluorophore that exhibits much lower phototoxicity than previous voltage indicators in cardiomyocyte monolayers, but suffers from low sensitivity to membrane potential changes. Here, we report that the addition of a single vinyl spacer in the fluorene molecular wire scaffold improves the voltage sensitivity 1.5- to 3.5-fold over fluorene-based voltage probes. Furthermore, we demonstrate the improved ability of the new vinyl-fluorene VoltageFluors to monitor action potential kinetics in both mammalian neurons and human-induced pluripotent stem cell-derived cardiomyocytes. Addition of the vinyl spacer between the aniline donor and fluorene monomer results in indicators that are significantly less phototoxic in cardiomyocyte monolayers. These results demonstrate how structural modification to the voltage sensing domain have a large effect on improving the overall properties of molecular wire-based voltage indicators.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos de Vinilo
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Miocitos Cardíacos
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Fluorenos
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Colorantes Fluorescentes
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Neuronas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article