H4K20me3 marks distal intergenic and repetitive regions in human mature spermatozoa.
Development
; 148(15)2021 08 01.
Article
en En
| MEDLINE
| ID: mdl-34345914
ABSTRACT
Sperm histones represent an essential part of the paternally transmitted epigenome, but uncertainty exists about the role of those remaining in non-coding and repetitive DNA. We therefore analyzed the genome-wide distribution of the heterochromatic marker H4K20me3 in human sperm and somatic (K562) cells. To specify the function of sperm histones, we compared all H4K20me3-containing and -free loci in the sperm genome. Sperm and somatic cells possessed a very similar H4K20me3 distribution H4K20me3 peaks occurred mostly in distal intergenic regions and repetitive gene clusters (in particular genes encoding odorant-binding factors and zinc-finger antiviral proteins). In both cell types, H4K20me3 peaks were enriched in LINEs, ERVs, satellite DNA and low complexity repeats. In contrast, H4K20me3-free nucleosomes occurred more frequently in genic regions (in particular promoters, exons, 5'-UTR and 3'-UTR) and were enriched in genes encoding developmental factors (in particular transcription activators and repressors). H4K20me3-free nucleosomes were also detected in substantial quantities in distal intergenic regions and were enriched in SINEs. Thus, evidence suggests that paternally transmitted histones may have a dual purpose:
maintenance and regulation of heterochromatin and guidance towards transcription of euchromatin.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Histonas
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Secuencias Repetitivas de Ácidos Nucleicos
Límite:
Humans
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Male
Idioma:
En
Año:
2021
Tipo del documento:
Article