Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016.
Arch Virol
; 166(11): 3165-3172, 2021 Nov.
Article
en En
| MEDLINE
| ID: mdl-34417874
ABSTRACT
This study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Mamastrovirus
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Infecciones por Astroviridae
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Gastroenteritis
Límite:
Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
País como asunto:
America do sul
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Brasil
Idioma:
En
Año:
2021
Tipo del documento:
Article