Insulin-Binding Peptide Probes Provide a Novel Strategy for Pancreatic ß-Cell Imaging.
Mol Pharm
; 18(12): 4428-4436, 2021 12 06.
Article
en En
| MEDLINE
| ID: mdl-34649437
ABSTRACT
Type 1 diabetes develops in childhood and adolescence, with peak incidence in the early teenage years. There is an urgent need for an accurate method to detect insulin-producing ß-cells in patients that is not affected by alterations in ß-cell function. As part of our research program to design specific probes to measure ß-cell mass, we recently developed a novel insulin-binding peptide probe (IBPP) for the detection of ß-cells in vivo. Here, we applied our innovative method to show specific labeling of this IBPP to human and mouse fixed ß-cells in pancreatic islets. Importantly, we showed staining of human and mouse islets in culture without any negative functional or cell viability impact. Moreover, the IBPP-stained mouse islets after tail vein injection in vivo, albeit with batch differences in staining efficiency. In conclusion, we provide evidence showing that the IBPP can be used for future accurate detection of ß-cell mass in a variety of preclinical models of diabetes.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Péptidos y Proteínas de Señalización Intracelular
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Diabetes Mellitus Tipo 1
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Células Secretoras de Insulina
Límite:
Animals
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article