Sample optimization for saliva 1H-NMR metabolic profiling.
Anal Biochem
; 640: 114412, 2022 03 01.
Article
en En
| MEDLINE
| ID: mdl-34656613
ABSTRACT
Nuclear Magnetic Resonance (NMR) based metabolomic analysis of whole saliva has provided potential diagnostic biomarkers for numerous human diseases contributing to a better understanding of their mechanisms. However, a comprehensive interpretation of the significance of metabolites in whole, parotid, and submandibular/sublingual saliva subtypes is still missing. Precision and reproducibility of sample preparation is an essential step. Here, we present a simple and efficient protocol for saliva 1H-NMR metabolic profiling. This procedure has been specifically designed and optimized for the identification and quantification of low concentration metabolites (as low as 1.1 µM) and is suitable for all the saliva subtypes.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saliva
Tipo de estudio:
Guideline
Idioma:
En
Año:
2022
Tipo del documento:
Article