Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.

Kwanten, Bert; Neggers, Jasper Edgar; Daelemans, Dirk

Kwanten, Bert; Neggers, Jasper Edgar; Daelemans, Dirk.

Kwanten B; KU Leuven Department of Microbiology, Immunology and Transplantation, Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Leuven, Belgium.
Neggers JE; KU Leuven Department of Microbiology, Immunology and Transplantation, Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Leuven, Belgium.
Daelemans D; Promakhos Therapeutics, Pagliuca Harvard Life Lab, Allston, Massachusetts, USA.

Methods Mol Biol ; 2377: 43-67, 2022.

Article en En | MEDLINE | ID: mdl-34709610

Asunto(s)

Sistemas CRISPR-Cas; Sistemas CRISPR-Cas/genética; ADN; Genes Esenciales; Mutagénesis; ARN Guía de Kinetoplastida/genética

Palabras clave

CRISPRres; CRISPRCas; Cas12a; Cas9; Drug target identification; Genetic screen; Resistance mutation; Target deconvolution; sgRNA tiling library

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas Tipo de estudio: Diagnostic_studies Idioma: En Año: 2022 Tipo del documento: Article

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas Tipo de estudio: Diagnostic_studies Idioma: En Año: 2022 Tipo del documento: Article