SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores.

ABSTRACT

ABBREVIATIONS ATG2 autophagy related 2; BECN1 beclin 1; COPII coat protein II; DMSO dimethyl sulfoxide; EBSS Earle's balanced salt solution; EGFP enhanced green fluorescent protein; ER endoplasmic reticulum; ERES ER exit site(s); GFP green fluorescent protein; H89 H-89 dihydrochloride hydrate; LAMP1 lysosomal-associated membrane protein 1; MAP1LC3/LC3 microtubule-associated protein 1 light chain 3; MTORC1 mechanistic target of rapamycin kinase complex 1; NS5A nonstructural protein 5A; PIK3C3/VPS34 phosphatidylinositol 3-kinase catalytic subunit type 3; PLA proximity ligation assay; PtdIns3P phosphatidylionositol-3-phosphate; RB1CC1/FIP200 RB1 inducible coiled-coil 1; RFP red fluorescent protein; RPS6KB1/S6K ribosomal protein S6 kinase B1; SBP streptavidin binding protein; SEC16A SEC16 homolog A, endoplasmic reticulum export factor; SEC31A SEC31 homolog A, COPII coat complex component; siRNA small interfering RNA; Str streptavidin; ULK1 unc-51-like autophagy activating kinase 1; VSVG vesicular stomatitis virus glycoprotein; WIPI2 WD repeat domain, phosphoinositide interacting 2; WT wild type.