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Soy diet induces intestinal inflammation in adult Zebrafish: Role of OTX and P53 family.
Micheloni, Giovanni; Carnovali, Marta; Millefanti, Giorgia; Rizzetto, Manuel; Moretti, Vittoria; Montalbano, Giuseppe; Acquati, Francesco; Giaroni, Cristina; Valli, Roberto; Costantino, Lucy; Ferrara, Fulvio; Banfi, Giuseppe; Mariotti, Massimo; Porta, Giovanni.
  • Micheloni G; Centro di Medicina Genomica, Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Carnovali M; IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
  • Millefanti G; Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Rizzetto M; Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Moretti V; Centro di Medicina Genomica, Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Montalbano G; Centro di Medicina Genomica, Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Acquati F; Centro di Medicina Genomica, Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Giaroni C; Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Valli R; Centro di Medicina Genomica, Department of Medicine and Surgery, University of Insubria, Varese, Italy.
  • Costantino L; Department of Molecular Genetics, Centro Diagnostico Italiano, Milano, Italy.
  • Ferrara F; Department of Molecular Genetics, Centro Diagnostico Italiano, Milano, Italy.
  • Banfi G; IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
  • Mariotti M; Vita-Salute San Raffaele University, Milan, Italy.
  • Porta G; IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
Int J Exp Pathol ; 103(1): 13-22, 2022 02.
Article en En | MEDLINE | ID: mdl-34725870
ABSTRACT
Inflammatory bowel diseases (IBDs) are a group of inflammatory conditions of the colon and small intestine, including Crohn's disease and ulcerative colitis. Since Danio rerio is a promising animal model to study gut function, we developed a soy-dependent model of intestinal inflammation in adult zebrafish. The soya bean meal diet was given for 4 weeks and induced an inflammatory process, as demonstrated by morphological changes together with an increased percentage of neutrophils infiltrating the intestinal wall, which developed between the second and fourth week of treatment. Pro-inflammatory genes such as interleukin-1beta, interleukin-8 and tumour necrosis factor alpha were upregulated in the second week and anti-inflammatory genes such as transforming growth factor beta and interleukin-10. Interestingly, an additional expression peak was found for interleukin-8 at the fourth week. Neuronal genes, OTX1 and OTX2, were significantly upregulated in the first two  weeks, compatible with the development of the changes in the gut wall. As for the genes of the p53 family such as p53, DNp63 and p73, a statistically significant increase was observed after two weeks of treatment compared with controls. Interestingly, DNp63 and p73 were shown an additional peak after four weeks. Our data demonstrate that soya bean meal diet negatively influences intestinal morphology and immunological function in adult zebrafish showing the features of acute inflammation. Data observed at the fourth week of treatment may suggest initiation of chronic inflammation. Adult zebrafish may represent a promising model to better understand the mechanisms of food-dependent intestinal inflammation.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glycine max / Enfermedades Inflamatorias del Intestino / Proteína p53 Supresora de Tumor / Dieta / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glycine max / Enfermedades Inflamatorias del Intestino / Proteína p53 Supresora de Tumor / Dieta / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article