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Cytokeratin 13 Is a New Biomarker for the Diagnosis of Limbal Stem Cell Deficiency.
Liang, Qingfeng; Le, Qihua; Wang, Leying; Cordova, Daniel; Baclagon, Elfren; Garrido, Sheyla González; Levin, Mary; Jin, Yusheng; Tseng, Chi-Hong; Rao, Jianyu; Deng, Sophie X.
  • Liang Q; Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Le Q; Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing, China.
  • Wang L; Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Cordova D; Department of Ophthalmology, Eye, Ear, Nose & Throat Hospital of Fudan University, Shanghai, China.
  • Baclagon E; Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing, China.
  • Garrido SG; Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Levin M; Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Jin Y; Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Tseng CH; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Rao J; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.
  • Deng SX; Department of Medicine, Statistic Core-General Internal Medicine and Health Service Research, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA; and.
Cornea ; 41(7): 867-873, 2022 Jul 01.
Article en En | MEDLINE | ID: mdl-34743102
ABSTRACT

PURPOSE:

The purpose of this study was to evaluate the expression of cytokeratin (K) 13 on the corneal surface and to validate its application in the diagnosis of limbal stem cell deficiency (LSCD).

METHODS:

This prospective comparative study included 26 corneal impression cytology (IC) specimens from patients diagnosed with LSCD. Twenty-three IC specimens from normal donors served as controls. K12 and K13 expression were detected on the IC specimens by immunohistochemistry study. The number of K12 + or K13 + cells in all areas of the IC was quantified using ImageJ software.

RESULTS:

The epithelial cells harvested from IC specimens from control corneas were all K12 + . In eyes with LSCD, K13 + and K12 + /K13 + cells accounted for 93.8% and 2.6%, respectively, in the cornea. In eyes with sectoral LSCD, the median number of K13 + cells in the clinically affected area was higher than that in the unaffected area (810.0 vs. 115.0 cells/mm 2 ; P < 0.001). No significant correlation was found between the LSCD severity and the number of K12 + cells (r = -0.284, P = 0.16) or K13 + cells (r = -0.011, P = 0.95). The presence of at least 16 K13 + cells/mm 2 was suggestive of LSCD.

CONCLUSIONS:

Identification of K13 + cells on IC specimens provides a simple and reliable method to detect conjunctival epithelial cells on the cornea. K13 is a marker for diagnosing LSCD and localizing the involved area in sectoral LSCD.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de la Esclerótica / Limbo de la Córnea / Epitelio Corneal / Enfermedades de la Córnea Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de la Esclerótica / Limbo de la Córnea / Epitelio Corneal / Enfermedades de la Córnea Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article