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Transcriptomic Studies Suggest a Coincident Role for Apoptosis and Pyroptosis but Not for Autophagic Neuronal Death in TBEV-Infected Human Neuronal/Glial Cells.
Fares, Mazigh; Gorna, Kamila; Berry, Noémie; Cochet-Bernoin, Marielle; Piumi, François; Blanchet, Odile; Haddad, Nadia; Richardson, Jennifer; Coulpier, Muriel.
  • Fares M; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Gorna K; MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK.
  • Berry N; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Cochet-Bernoin M; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Piumi F; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Blanchet O; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Haddad N; CHU Angers, Centre de Ressources Biologiques, BB-0033-00038 Angers, France.
  • Richardson J; UMR BIPAR 956, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
  • Coulpier M; UMR1161 Virologie, Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, F-94700 Maisons-Alfort, France.
Viruses ; 13(11)2021 11 10.
Article en En | MEDLINE | ID: mdl-34835061
ABSTRACT
Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, Flavivirus genus, is responsible for neurological symptoms that may cause permanent disability or death. With an incidence on the rise, it is the major arbovirus affecting humans in Central/Northern Europe and North-Eastern Asia. Neuronal death is a critical feature of TBEV infection, yet little is known about the type of death and the molecular mechanisms involved. In this study, we used a recently established pathological model of TBEV infection based on human neuronal/glial cells differentiated from fetal neural progenitors and transcriptomic approaches to tackle this question. We confirmed the occurrence of apoptotic death in these cultures and further showed that genes involved in pyroptotic death were up-regulated, suggesting that this type of death also occurs in TBEV-infected human brain cells. On the contrary, no up-regulation of major autophagic genes was found. Furthermore, we demonstrated an up-regulation of a cluster of genes belonging to the extrinsic apoptotic pathway and revealed the cellular types expressing them. Our results suggest that neuronal death occurs by multiple mechanisms in TBEV-infected human neuronal/glial cells, thus providing a first insight into the molecular pathways that may be involved in neuronal death when the human brain is infected by TBEV.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neuroglía / Apoptosis / Virus de la Encefalitis Transmitidos por Garrapatas / Piroptosis / Neuronas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2021 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neuroglía / Apoptosis / Virus de la Encefalitis Transmitidos por Garrapatas / Piroptosis / Neuronas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2021 Tipo del documento: Article