Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method.
Sensors (Basel)
; 21(23)2021 Nov 24.
Article
en En
| MEDLINE
| ID: mdl-34883813
ABSTRACT
Four serotypes of dengue virus (DENV), type 1 to 4 (DENV-1 to DENV-4), exhibit approximately 25-40% of the difference in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from specimens followed by PCR amplification is the current standard method of DENV serotype determination. However, since this method is time-consuming, rapid detection systems are desirable. We established several mouse monoclonal antibodies directed against DENV non-structural protein 1 and integrated them into rapid DENV detection systems. We successfully developed serotype-specific immunochromatography systems for all four DENV serotypes. Each system can detect 104 copies/mL in 15 min using laboratory and clinical isolates of DENV. No cross-reaction between DENV serotypes was observed in these DENV isolates. We also confirmed that there was no cross-reaction with chikungunya, Japanese encephalitis, Sindbis, and Zika viruses. Evaluation of these systems using serum from DENV-infected individuals indicated a serotype specificity of almost 100%. These assay systems could accelerate both DENV infection diagnosis and epidemiologic studies in DENV-endemic areas.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Dengue
/
Virus del Dengue
/
Virus Zika
/
Infección por el Virus Zika
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article