RNA circles with minimized immunogenicity as potent PKR inhibitors.
Mol Cell
; 82(2): 420-434.e6, 2022 01 20.
Article
en En
| MEDLINE
| ID: mdl-34951963
ABSTRACT
Exon back-splicing-generated circular RNAs, as a group, can suppress double-stranded RNA (dsRNA)-activated protein kinase R (PKR) in cells. We have sought to synthesize immunogenicity-free, short dsRNA-containing RNA circles as PKR inhibitors. Here, we report that RNA circles synthesized by permuted self-splicing thymidylate synthase (td) introns from T4 bacteriophage or by Anabaena pre-tRNA group I intron could induce an immune response. Autocatalytic splicing introduces â¼74 nt td or â¼186 nt Anabaena extraneous fragments that can distort the folding status of original circular RNAs or form structures themselves to provoke innate immune responses. In contrast, synthesized RNA circles produced by T4 RNA ligase without extraneous fragments exhibit minimized immunogenicity. Importantly, directly ligated circular RNAs that form short dsRNA regions efficiently suppress PKR activation 103- to 106-fold higher than reported chemical compounds C16 and 2-AP, highlighting the future use of circular RNAs as potent inhibitors for diseases related to PKR overreaction.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
EIF-2 Quinasa
/
Inhibidores de Proteínas Quinasas
/
ARN Circular
Límite:
Humans
Idioma:
En
Año:
2022
Tipo del documento:
Article