Impact of limb amputation and cisplatin chemotherapy on metastatic progression in mouse models of osteosarcoma.
Sci Rep
; 11(1): 24435, 2021 12 24.
Article
en En
| MEDLINE
| ID: mdl-34952927
ABSTRACT
Development of animal models that accurately recapitulate human cancer is an ongoing challenge. This is particularly relevant in the study of osteosarcoma (OS), a highly malignant bone tumor diagnosed in approximately 1000 pediatric/adolescent patients each year. Metastasis is the leading cause of patient death underscoring the need for relevant animal models of metastatic OS. In this study, we describe how existing OS mouse models can be interrogated in a time-course context to determine the kinetics of spontaneous metastasis from an orthotopically implanted primary tumor. We evaluated four highly metastatic OS cell lines (3 human, 1 mouse) to establish a timeline for metastatic progression in immune deficient NSG mice. To discern the effects of therapy on tumor development and metastasis in these models, we investigated cisplatin therapy and surgical limb amputation at early and late timepoints. These data help define the appropriate observational periods for studies of metastatic progression in OS and further our understanding of existing mouse models. Efforts to advance the study of metastatic OS are critical for facilitating the identification of novel therapeutics and for improving patient survival.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
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Osteosarcoma
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Cisplatino
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Extremidades
Límite:
Animals
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Humans
Idioma:
En
Año:
2021
Tipo del documento:
Article