Characterization and function of mandarin fish c-Myc during viral infection process.
Fish Shellfish Immunol
; 120: 686-694, 2022 Jan.
Article
en En
| MEDLINE
| ID: mdl-34968711
ABSTRACT
c-Myc is a transcription factor and master regulator of cellular metabolism, and plays a critical role in virus replication by regulating glutamine metabolism. In this study, the open-reading frame (ORF) of c-Myc, designated as Sc-c-Myc, was cloned and sequenced. Multiple alignment of the amino acid sequence showed that the conserved domain of Sc-c-Myc, including the helix-loop-helix-zipper (bHLHzip) domain and Myc N-terminal region, shared high identities with other homologues from different species. Sc-c-Myc mRNA was widely expressed in the examined tissues of mandarin fish, and the higher mRNA levels was expressed in hind kidney. Moreover, mRNA and protein level of Sc-c-Myc was significantly increased in the Chinese perch brain (CPB) cells and spleen of mandarin fish post infection with infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV). Sc-c-Myc overexpression promoted ISKNV and SCRV replication, on the contrary, knocking down Sc-c-Myc restrained ISKNV and SCRV replication. These results indicated that Sc-c-Myc involved in ISKNV and SCRV replication and proliferation, providing a potential target for the development of new therapic strategy against ISKNV and SCRV.
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Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Perciformes
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Proteínas Proto-Oncogénicas c-myc
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Infecciones por Virus ADN
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Enfermedades de los Peces
Límite:
Animals
Idioma:
En
Año:
2022
Tipo del documento:
Article