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Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library.
Miyamoto, Naoki; Go, Young-Hyun; Ciaramicoli, Larissa Miasiro; Kwon, Haw-Young; Kim, Heon Seok; Bi, Xuezhi; Yu, Young Hyun; Kim, Beomsue; Ha, Hyung-Ho; Kang, Nam-Young; Yun, Seong-Wook; Kim, Jin-Soo; Cha, Hyuk-Jin; Chang, Young-Tae.
  • Miyamoto N; Center for Self-assembly and Complexity, Institute for Basic Science (IBS) Pohang 37673 Republic of Korea ytchang@postech.ac.kr.
  • Go YH; Department of Life Science, Sogang University 35 Baekbeom-ro Mapo-gu Seoul 04107 South Korea.
  • Ciaramicoli LM; Department of Chemistry, Pohang University of Science and Technology (POSTECH) Pohang Gyeongbuk 37673 Republic of Korea.
  • Kwon HY; Center for Self-assembly and Complexity, Institute for Basic Science (IBS) Pohang 37673 Republic of Korea ytchang@postech.ac.kr.
  • Kim HS; Division of Oncology, Department of Medicine, Stanford University School of Medicine Stanford CA USA.
  • Bi X; Bioprocessing Technology Institute, Agency for Science, Technology and Research (ASTAR) Singapore 138668 Singapore.
  • Yu YH; College of Pharmacy, Sunchon National University Jungangro 255 Sunchon 57922 South Korea.
  • Kim B; Neural Circuit Research Group, Korea Brain Research Institute (KBRI) Daegu 41068 Republic of Korea.
  • Ha HH; College of Pharmacy, Sunchon National University Jungangro 255 Sunchon 57922 South Korea.
  • Kang NY; Department of Convergence IT Engineering, Pohang University of Science and Technology Pohang Gyeongbuk 37673 Korea.
  • Yun SW; Nonclinical Drug Safety, Boehringer Ingelheim Pharma GmbH & Co. KG 88397 Biberach an der Riss Germany.
  • Kim JS; Department of Chemistry, Seoul National University Seoul 08826 Republic of Korea.
  • Cha HJ; Center for Genome Engineering, Institute for Basic Science (IBS) Daejeon 34126 Republic of Korea.
  • Chang YT; College of Pharmacy, Seoul National University Seoul 08826 Republic of Korea hjcha93@snu.ac.kr.
RSC Chem Biol ; 2(6): 1590-1593, 2021 Dec 02.
Article en En | MEDLINE | ID: mdl-34977573
ABSTRACT
CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Año: 2021 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Año: 2021 Tipo del documento: Article