Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins.
Nat Biotechnol
; 40(5): 779-786, 2022 05.
Article
en En
| MEDLINE
| ID: mdl-34980910
ABSTRACT
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in response to blue light irradiation. When fused to various RNA effectors, LicV allows for optogenetic control of RNA localization, splicing, translation and stability in cell culture. Furthermore, LicV-assisted CRISPR-Cas systems allow for efficient and tunable photoswitchable regulation of transcription and genomic locus labeling. These data demonstrate that the photoswitchable RBP LicV can serve as a programmable scaffold for the spatiotemporal control of synthetic RNA effectors.
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1
Banco de datos:
MEDLINE
Asunto principal:
ARN
/
Optogenética
Idioma:
En
Año:
2022
Tipo del documento:
Article